Bill Gates Essays (575 words) - Big History, Bill Gates,

Bill Gates William H. Gates Chairman and Chief Executive Officer Microsoft Corporation William (Bill) H. Gates is chairman and chief executive officer of Microsoft Corporation, the leading provider, worldwide, of software for the personal computer. and employs more than 20,000 people in 48 countries. Born on October 28, 1955, Gates and his two sisters grew up in Seattle. Their father, William H. Gates II, is a Seattle attorney. Their late mother, Mary Gates, was a schoolteacher, University of Washington regent and chairwoman of United Way International. Gates attended public elementary school and the private Lakeside School. There, he began his career in personal computer software, programming computers at age 13. In 1973, Gates entered Harvard University as a freshman, where he lived down the hall from Steve Ballmer, now Microsoft's executive vice president for sales and support. While at Harvard, Gates developed the programming language BASIC for the first microcomputer -- the MITS Altair. In his junior year, Gates dropped out of Harvard to devote his energies to Microsoft, a company he had begun in 1975 with Paul Allen. Guided by a belief that the personal computer would be a valuable tool on every office desktop and in every home, they began developing software for personal computers. Gates' foresight and vision regarding personal computing have been central to the success of Microsoft and the software industry. Gates is actively involved in key management and strategic decisions at Microsoft, and plays an important role in the technical development of new products. A significant portion of his time is devoted to meeting with customers and staying in contact with Microsoft employees around the world through e-mail. Under Gates' leadership, Microsoft's mission is to continually advance and improve software technology and to make it easier, more cost-effective and more enjoyable for people to use computers. In 1995, Gates wrote The Road Ahead, his vision of where information technology will take society. Co-authored by Nathan Myhrvold, Microsoft's chief technology officer, and Peter Rinearson, The Road Ahead held the No. 1 spot on the New York Times' bestseller list for seven weeks. Published in the U.S. by Viking, the book was on the NYT list for a total of 18 weeks. Published in more than 20 countries, the book sold more than 400,000 copies in China alone. In 1996, while redeploying Microsoft around the Internet, Gates thoroughly revised The Road Ahead to reflect his view that interactive networks are a major milestone in human history. The paperback second edition has also become a bestseller. Gates is donating his proceeds from the book to a non-profit fund that supports teachers worldwide who are incorporating computers into their classrooms. In addition to his passion for computers, Gates is interested in biotechnology. He sits on the board of the Icos Corporation and is a shareholder in Darwin Molecular, a subsidiary of British-based Chiroscience. He also founded Corbis Corporation, which is developing one of the largest resources of visual information in the world-a comprehensive digital archive of art and photography from public and private collections around the globe. Gates also has invested with cellular telephone pioneer Craig McCaw in Teledesic, a company that is working on an ambitious plan to launch hundreds of low-orbit satellites around the globe to provide worldwide two-way broadband telecommunications service. In the decade since Microsoft has gone public, Gates has donated more than $270 million to charities, including $200 million to the William H. Gates Foundation. The focus of Gates' giving is in three areas: education, population issues and access to technology. Gates was married on Jan. 1, 1994 to Melinda French Gates. They have one child, Jennifer Katharine Gates, born in 1996.

Impact of New Economic Policy 1991 Essays

Impact of New Economic Policy 1991 Essays Impact of New Economic Policy 1991 Essay Impact of New Economic Policy 1991 Essay Essay Topic: Claim of Policy Industrialization through import permutation and public sector production with accent on heavy industry has been a really of import aim of our planning for development. In peculiar an of import differentiation was made among industries to be developed entirely by the populace sector. those reserved for the private sector. and those unfastened to development by either or both sectors. The reforms of 1991 abolished industrial licensing. except in a few industries for locational grounds or for environmental considerations. and import licensing. except in the instance of most consumer goods. Restrictions under the Monopolies and Restrictive Trade Practices Act were eased. Entry demands ( including bounds on equity engagement ) for foreign direct investing were relaxed. private ( domestic and foreign ) investing were allowed into sectors such as power which had been reserved for public sector investing merely. Disinvestment of equity in the populace sector was besides initiated. The reforms. by concentrating chiefly on the private sector and non turn toing the jobs of PSEs. have exacerbated them. Industry histories for 28 % of the GDP and use 14 % of the entire work force. [ 20 ] In absolute footings. India is 12th in the universe in footings of nominal mill end product. [ 77 ] The Indian industrial sector underwent important alterations as a consequence of the economic reforms of 1991. which removed import limitations. brought in foreign competition. led to denationalization of certain public sector industries. liberalized the FDI government. improved substructure and led to an enlargement in the production of fast traveling consumer goods. [ 78 ] Post-liberalization. the Indian private sector was faced with increasing domestic every bit good as foreign competition. including the menace of cheaper Chinese imports. It has since handled the alteration by squashing costs. revamping direction. and trusting on inexpensive labor and new engineering. However. this has besides reduced employment coevals even by smaller makers who earlier relied on comparatively labor-intensive procedures. [ Decision Over the decennaries. the Indian theoretical account of development has created what is called the double economic system. On the one manus an enclave of big urban industries based on modem engineering was created both in the private and public sectors. uhich rernain tied to foreign assistance and engineering. On the other manus. there was the remainder of the economic system of the hapless which was left to fend for itself. I’he financial and trade systems were besides designed to enclave the economic system. rhe new economic policy will surely beef up this dichotomy and make something wasteful besides. which is evidently anti-poor. On behalf of the new industrial policy it is claimed that it will let go of the Indian industry from unneeded bureaucratic bonds by cut downing the n~lmber of clearances required from the Government. Chiefly. there are two concession~s: The proposed policy allows foreign investing up to 1 per cent to 100 per cent equity on automatic footing topic to some limitations on imports of capital goods. This grant is meant merely for the aliens or those who collaborate with Indian opposite numbers. In other words this proviso will beef up the clasp of the foreign companies on Indian Industry. I h e 2nd grant is for the Indian enterprisers and relates to technological imports. The policy provides no defence against inauspicious impact on the domestic capital goods industry. There is no selectivity in the policy I n the sense that import should non be allowed in those instances where domestic market is in a place to provide capital goods in equal measure and quality. There is no appropriate industry program with appropriate industrial mix. technological choice on the footing of precedences. 22 The sudden displacement from import permutation to export publicity misses both the co~nplementarity and the sequence. It besides misses the demand to take monolithic deformation: j. dependence and the ruptures between the two. The basic standard finding import permutation and taking industrial undertakings has so Ear aimed at salvaging foreign exchange in the short and average periods. Some projections were made for long-run foreign exchange demands but there was Small consideration given to the fact that short-tenn additions in ioreign exchange secured through puting up of all sorts of precedence every bit good as non-priority industries either for limited export or import permutation cnight lead to greater dependance on the universe market and foreign capital imd this push India into a more serious external fiscal crisis. Of class. non all industries set up were that sort. Quite a few. peculiarly the basic and natural stuff bring forthing undertakings. had long term good effects but a still larger figure did non fall in this class. Indeed. trust on foreign coaction and capital and engineering every bit good as universe market and universe monopolies have led both to greater dependance on exterior every bit good as greater and spread outing influence of external capital on Indian industry. peculiarly the new industries. which were set up with the professed intent of making economic independency. What came as an unintended effect of old policy will now be accentuated as a effect of N E I . ~ ~ The new policy is wholly soundless on employment. During the 1880ss. when the industrial growtli increased from five per cent to eight per cent. employment snaps uniformly declined in all. except in the services sector. Unemployment is no A ; going politically unacceptable and already taking to monolithic societal agitation. One expected of the Government to do a clear statement on the employment aim. peculiarly when there is traveling to be a monolithic displacement towards ask foring foreign capital which will be invested merely in capital- intensive industries. Modemisation and export publicity will escalate capital strength every bit good as import-intensity which is besides biased in favc~ur of capital and against labor. The agricultural sector of the economic system is adversely affected by the New Economic Policy. Our farming community is now at the clemency of transnational corporations. They were now confronting two types of jobs. On one side the cost of cultivation is increasing as a consequence of retreating subsidy by the authorities to farm inputs. and the other side they were non acquiring compensable monetary value for their merchandises. The disregard and jobs of a sector which provide support to more than 60 % of the population is black to the Indian economic system. Most of the agricultural harvests shows a worsening growing rate after the acceptance of the New Economic Policy. The economic or. more specifically fiscal crisis is non to the full independent. I t is linked. both as a cause and consequence with many other crisis. The whole society is caught with Inany fold societal paroxysms. The NEP is a despairing dip to rneet some immediate economic menaces. It may or may non win. It has positive facets which are welcome but there are many others which may intensify the crisis. If monolithic investing in the populace sector and import permutation failed to do lndia autonomous how can private sector. including foreign investors and export publicity achieve autonomy under imposed external restraints? So it is logical to reason that the way of the economic system has to alter. There ought to be paradigm displacements towards a more self reliant. sustainable and merely development theoretical account whose end will besides be fundamentally different: non more production of material richness but the creative activity of a new single and society- a contented comfortable community based on a set of values Gandhi propagated and worked for viz. . co-operation sharing engagement common authorization non-violence and peace prior to the mid-1960s India relied on imports and nutrient assistance to run into domestic demands. However. two old ages of terrible drouth in 1965 and 1966 convinced India to reform its agricultural policy. and that India could non trust on forei gn assistance and foreign imports for nutrient security. India adopted important policy reforms focused on the end of foodgrain autonomy. This ushered in India’sGreen Revolution. It began with the determination to follow superior giving up. disease immune wheat assortments in combination with better farming cognition to better productiveness. India ranks 2nd worldwide in farm end product. Agriculture and allied sectors like forestry. logging and fishing accounted for 15. 7 % of the GDP in 2009–10. employed 52. 1 % of the entire work force. and despite a steady diminution of its portion in the GDP. is still the largest economic sector and a important piece of the overall socio-economic development of India. [ 94 ] Outputs per unit country of all harvests have grown since 1950. due to the particular accent placed on agribusiness in the five-year programs and steady betterments in irrigation. engineering. application of modern agricultural patterns and proviso of agricultural recognition and subsidies since the Green Revolution in India. However. international comparings reveal the mean output in India is by and large 30 % to 50 % of the highest mean output in the universe. [ 95 ] Indian provinces Uttar Pradesh. Punjab. Haryana. Madhya Pradesh. Andhra Pradesh. Bihar. West Bengal. Gujarat and Maharashtra are cardinal agricultural lending provinces of India. India receives an mean one-year rainfall of 1. 208 millimeters ( 47. 6 in ) and a entire one-year precipitation of 4000 billion three-dimensional meters. with the entire utilisable H2O resources. including surface andgroundwater. amounting to 1123 billion three-dimensional meters. [ 96 ] 546. 820 square kilometers ( 211. 130 sq myocardial infarction ) of the land country. or about 39 % of the entire cultivated country. is irrigated. [ 97 ] India’s inland H2O resources including rivers. canals. pools and lakes and marine resources consisting the E and west seashores of the Indian ocean and other gulfs and bays provide employment to about six million people in the piscaries sector. In 2008. India had the world’s 3rd largest fishing industry. [ 98 ] India is the largest manufacturer in the universe of milk. jute and pulsations. and besides has the world’s 2nd largest cowss population with 175 million animate beings in 2008. [ 99 ] It is the 2nd largest manufacturer of rice. wheat. sugar cane. cotton and Indian potatos. every bit good as the 2nd largest fruit and vegetable manufacturer. accounting for 10. 9 % and 8. 6 % of the universe fruit and vegetable production severally. [ 99 ] India is besides the 2nd largest manufacturer and the largest consumer of silk in the universe. bring forthing 77. 000 million dozenss in 2005. [ 100 ]

Early Childhood Education Program Term Paper Example | Topics and Well Written Essays - 2250 words

Early Childhood Education Program - Term Paper Example This center has a goal of providing the best training and education for eight hours a day operation. For ages three to five years old, the curriculum will be conducted for four hours and five days a week. This is to ensure that the development of the children of this age will be observed. This center is very essential to provide the positive impact and nurture on the children within the community. As a center designed to provide quality education for infants and toddlers as well as preschoolers, this school is important in the community. Vision To be the best in providing the children the finest life and career skills using the comprehensive classroom technology plan. To be committed to provide true service where teachers and educators are knowledgeable and responsible and who truly care to their students. We believe that we can offer and develop the learning and innovation skills of the students. We desire to inspire to our valued students the importance of learning the information, media and technology skills by providing the best classroom environment and teaching plan that suits every need of the students. We dedicate all our gifts, efforts and power to its realization. With God’s help, this vision will be upheld. ...To be able to instil the value of classroom development and provide the best learning environment to the students for the common good. Philosophy Children are the most precious gifts to parents. For a teacher, a second most valuable guide to each student, teaching is a very essential job that contributes to intellectual growth and future of a child. Subjects and activities included in each curriculum are expertise of the teacher. The students should be moulded into active participants of a learning process through classrooms equipped to teach the students and a well organized curriculum to fit the needs of the students. In the end, the students should meet the premiere standards and assessments. The proposed vision, mission and philosoph y are based on the Constructivist Theory of child development and learning. This theory was popularized by Jean Piaget, Maria Montessori and Lev Vygotsky who believed that interaction of young children with their environment and their peers greatly influence their learning and development (Theories of Child Development and Learning, n.d., par.6). According to this theory, it is thus important that physical environment and curriculum must be given importance and utmost groundwork. Thus in the vision, mission and philosophy, it has been reiterated that the best classroom environment and curriculum must be provided as fundamentals for excellent education. The stated vision, mission and philosophy were founded on my belief that education is the foundation of a child’s learning and intellectual growth. Thus, teachers must be capable of the expertise when instilling values and learning to children.

Comparison of a Poem to a Song Coursework Example | Topics and Well Written Essays - 1250 words

Comparison of a Poem to a Song - Coursework Example The poem â€Å"theme for English B† is a poem written at the peak of racial discrimination in the year 1951.  In the poem,  Ã¢â‚¬Å"theme for English B† Hughes talks about the differences between him and his class instructor (Bloom, pg 21). The poet talks about being the only colored person in his  class  and shows the  feeling  of his  likeness  to other races, solely â€Å" white† and yet different throughout the poem. Using his  community  (Harlem) as an  inspiration  Hughes manages to write a poem that depicts his  ethnic  affiliation. The class  instructor  gave a  precise  brief description of the  assignment  that is, go home, write a page tonight, and let the  page  be the  original  thoughts of students (Fetzer, pg 23). The persona in the  poem  remains  uncertain  on whether the task is that  easy  as portrayed by the class instructor.  Afterwards,  the student outlines the reasons substantia ting his  thought  that such an  assignment  could not be  easy. The speaker in the poem â€Å"theme for English B† is older compared to most white students in the English class. Secondly, the student gives the  route  he takes from  school  to his  residence  where the narrator sits  to do  his  assignment (Bloom, pg 46). The student perceives the  difficulty in writing true ideals or something that is only purported to be true. This judgment was at the discretion of the  white  class  instructor. The student plunges into listing what he likes, subsequently supposing that being colored does not make him different from the things he likes as other races.  The speaker in the  poem  is bothered  if his black identity  will make a difference in what he writes (Fetzer, pg 44). Secondly,  he wonders whether he will be able  to talk  with a white tutor  given that  he  is black. Despite these ill feelings, the speaker in the   poem  finally admits that both of them are part of each other. The speaker then delves into an  extremely  significant  conclusion  that as the black students learn  from the  white tutor, the  instructor  equally  learns from the  black student.  The tone of Langston Hughes in his poem befits the  description  of  mildly  ironical’ as he shows his difficulty in  earnestly  communicating  his own feelings at the  specifications  of his  white  tutor of English.  Irony is  manifest  in the  poem  where he commences by saying â€Å"I wonder if it is that simple,† but at the end of the poem, he succeeds in doing just that in a  remarkably  correct  manner. His actual inner being comprises of two distinguishing aspects; he is black and very different from the instructor of his class. Secondly, he is an American just like every other American inclusive of his tutor. The author of the poem embraces a conciliat ory tone that acknowledges the fact that the two races are part of each other. Nevertheless, the poem ends on a tone full of  regret  somewhat more  free. Besides poems, songs are also excellent mediums of creating awareness or sensitizing the  society. An example is the  song  we shall be  free  by Garth Brooks. The  song  Ã¢â‚¬Å"We Shall Be Free† is a song produced by American country music artist during the year 1992. The artist was born on February 7, 1962 in America. The artist in the release of this song collaborated with Stephanie Davis. In this song, an ordinary  man  conceives of a world in which human beings are free from earthly subjugations. Topics addressed in this

Time series analysis of stock price Research Proposal

Time series analysis of stock price - Research Proposal Example 6). Stock refers to a fraction of ownership of a company by a person. It is a subdivision of a company’s capital that is sold to interested investors for capital generation. Based on the terms of the sale, stock, also known as shares are transferable at timely market prices (Borrowski, p. 3). Capital market is a network in which shares are sold. It consists of companies that issue the stock, the stocks to be traded and the investors who buy or sell the stock at a particular time (Borrowski, p. 3). This research proposal has been motivated by the increasing essence of capital markets in economies and has further been facilitated by the perception that statistics can be used to understand factors in stock prices such as trend and seasonality. The paper with this respect seeks to conduct a time series analysis of stock prices in the New York Stock Exchange market. It will explore some of the factors that affect stock prices and in light with analysis of stock prices of listed companies in a sector, move to investigate possible factors that affects trend and seasonality factors of stock prices in the financial sector’s New York Stock Exchange market. The stock exchange markets, as a forum for buying and selling of shares of companies operates like the normal markets in terms of financial implications of transactions. This means that among other factors, buyers and sellers engage in transactions with the aims of either buying the shares for as low costs as possible or selling them at as high prices as possible. Forces of demand and supply, subject the perceived financial position of particular companies that owns the shares, therefore influences the prices of shares over time. Stock prices are therefore expected to be on high demand when investors predicts higher stability and returns from investing in the issuing company. Similarly, factors that would affect the ability of investors to spend in the capital market will also generally dictate the trend

Effect Of Growth Factors Fcs On Cell Proliferation Biology Essay

Effect Of Growth Factors Fcs On Cell Proliferation Biology Essay Introduction: The technique in which cells normally of one type, removed from animal tissue are grown in presence of supplements and growth factors under controlled conditions is called Cell Culturing. Different cells require different conditions of temperature and gas for their appropriate growth in a cell incubator moreover growth media plays a crucial role in cell culturing, which can vary in glucose concentration, pH, growth factors like fetal calf serum (FCS). The effect of one of the above mentioned variables, we are going to study in this experiment is growth factors. Growth factors as the name suggests enhancers the growth which supplements the media are obtained from animal blood such as calf serum. Cell proliferation is studied by various methods, mainly used methods involve measuring the colour intensity taken up by DNA of cell during cell proliferation in presence of staining dyes e.g. crystal violet and MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide). The aim of this experiment is to study cell proliferation by using crystal violet and MTT methods while using mouse Y1 adrenocortical cells under the effect of growth factor FCS. Mouse Y1 adrenocortical cell are grown in Dulbeccos modification of Eagles medium as monolayer cultures. Crystal Violet: It is biological stain used in cell proliferation method which stains DNA of the cells after permeabilisation with methanol. The positive ammonium ions of crystal violet stain DNA of cells in blue colour. The colour intensity produced is directly proportional to number of cells, which is determined in stained cells by colorimeter. Staining with crystal violet and MTT are based on principal of absorbance. MTT: The MTT assay is a standard colorimetric assay in which activity of reductase enzymes are measured which convert MTT to blue insoluble formazan. The metabolic activity of viable cells is used as basic parameter in MTT assay. The tetrazolium salt, MTT is now widely used in quantitative measurement of cell proliferation. This method depends upon uptake of MTT by viable cells, which is a water soluble tetrazolium salt producing a yellowish solution. MTT is cleaved to insoluble blue formazan in the mitochondria of living cells by succinic dehydrogenases in the mitochondrial respiratory chain. Material Used: Mouse Y1 adrenocortical cells, Dulbeccos medication of Eagles medium (DMEM) containing 10% Fetal calf serum, 2mM glutamine, 100u/ml penicillin and 100mg/ml streptomycin, crystal violet stain (0.1% solution in 200mM boric acid), MTT (5mg/ml MTT solution in PBS), PBS methanol, 10% glacial acetic acid, distilled water, acid-isopropanol. Apparatus used: Laminar flow cabinet sterilized two 96 well plates, multi well pipettes, pasture pipettes, sterilized T-flasks, sterilised empty reservoirs, gas incubator, fume cupboard, spectrophotometer etc. Method: Cells of mouse Y1 adrenocortical were separated from their substratum with tris in EDTA as they grow in monolayer cultures. Then added same volume of medium and centrifuged after that number of cells were counted on haemocytometer and diluted to concentration of 1.25 à ¢Ã¢â‚¬ ¦Ã‚ © 105 cells/ml and made it up to 30 ml. Then cells were passaged into centre 60 wells of 96 well plate in duplicate with concentration of 0.25 à ¢Ã¢â‚¬ ¦Ã‚ © 10à ¢Ã‚ Ã‚ µ cells/ 200ÃŽÂ ¼l in each well while outside wells of 96 well plate were filled with same amount of phosphate buffered saline (PBS) and allowed the cells to incubate overnight at 37  °C temperature in humidified gas incubator. After that cells were washed with PBS three times and different wells of each plate were treated with different concentrations of FCS which is shown in table 1. Hence 12 wells of each plate were treated with 0, 1, 5, 10 and 20% v/v concentration of FCS and both plates were incubated for 72 hours. One plate was used for crystal violet staining method and other for MTT assay. Table 1: Dilution of FCS % FCS Volume of Serum (ml) Volume of Medium (ml) 0 0 10 1 0.1 9.9 5 0.5 9.5 10 1 9 20 2 8 Crystal violet staining method: For this method cell media was removed first of all from incubated plate and then cells were washed with PBS. After that were fixed with 200ÃŽÂ ¼l of methanol for 15 minutes in fume cupboard. Then methanol were removed and cells were allowed to dry in fume cupboard for few minutes. Then cells were treated for 20 minutes with crystal violet stain 200ÃŽÂ ¼l/well. Later cells were washed three times with distilled water and stained cell layer was allowed to solubilised in the 50ÃŽÂ ¼l of 10% glacial acetic acid and plates were incubated for 30 minutes in gas incubator. After that absorbance of each well was read by plate reader spectrophotometer set at 540nm. MTT Method: To perform MTT assay, each of centre 60 wells of 96 well plate was treated with 20ÃŽÂ ¼l of MTT solution and plate was incubated for 4 hours at 37o C temperature in gas incubator. After 4 hours, the medium was removed from each well and 100ÃŽÂ ¼l of acid-isopropand was added to dissolve blue formazan crystal in the cell layer. Then plate was incubated for 30 minutes at room temperature. When blue formazan crystal were solubilised, absorbance of each well was measured at 570nm using the plate reader. Calculations: Cells in five squares of Haemocytometer = 24 Volume of each square is =4ÃÆ'-10-3ÃŽÂ ¼l The no. of cells in five squares multiplied with 5ÃÆ'-104 gives no. of cells in 1ml. Hence no. of cell in 1ml = 1.2ÃÆ'-106 cells/ml Required cell suspension = 1.25ÃÆ'-105 cells/ml Dilution Factor = Concentration Required/Concentration got Dilution Factor = 0.104 Therefore, in order to make 30 ml of cell suspension 3.125 ml of cell suspension was mixed with 26.875 ml of medium. Similarly 30 ml of cell suspension was prepared having 1.25ÃÆ'-105 cells/ml. Results: Observation Table for crystal violet staining method %FCS Average Standard Deviation 0 0.205 0.0218 1 0.255 0.0307 5 0.309 0.0450 10 0.432 0.0614 20 0.581 0.0844 Table2: Data obtain from plate reader at 540nm Graph showing effect of FCS with Crystal Violet Method Above graph shows that with increase in serum concentration the absorbance increases, which is directly proportional to cell number. Observation Table of MTT staining method: % FCS Average Standard Deviation 0 0.094 0.0081 1 0.115 0.0138 5 0.152 0.0191 10 0.276 0.0724 20 0.400 0.0807 Table3: Data obtained from plate reader at 570nm. Graph showing effect of FCS with MTT staining Above graph shows that with increase in serum concentration the absorbance increases, which is directly proportional to cell number. Discussion: Crystal Violet Staining method and MTT Assay is based on principle of absorbance, more is colour intensity, more will be the absorbance value. The result of Crystal violet staining method clearly indicated that absorbance value was directly proportional to cell proliferation as it was increasing with concentration of FCS. FCS stimulated Cell Proliferation Result in more cells and DNA Methanol increased cell membrane permeability Result in more stained DNA More Colour intensity Hence More Absorbance Value Similar results were seen in MTT Assay but in this assay only viable cells were stained while in crystal violet method both viable and non viable cells were stained. So Crystal Violet method of staining is not specific staining technique because in this absorbance is not direct index of cell viability. The drawback of MTT Assay is that some reducing agent may reduce MTT also which could show slight increase in absorbance, moreover this method depends on some variable like pH, presence of D-glucose and pyridine nucleotides which can affect the specificity of Assay. In spite of above said limitations these methods are mostly followed because they are safe, simple, cheap and reproducible. Differentiation of K562 cells to megakaryocytes/platelets To study cell differentiation of K652 cells chronic myelogenous leukaemia, K652 cell line, indicates an early differentiation stage of granulocyte lineage. K652 cells are non-adherent, circular shaped with small microvilli. In the presence of tumour promoters like phorbol myristate acetate (PMA) these type of cell are differentiated to megakaryocytes. The induction of megakaryocytic differentiation of K652 cells is known to be initiated by two signalling pathways which are the nuclear factor kappa B(NF-ÃŽÂ ºB) -depends pathways and other is extracellular signal regulated kinase (ERK)/mitogen activated kinase (MAPK) dependent pathways. Human chromic myelogenous leukemic cells, K652 cells have Philadelphia chromosome. Tumour promoter, PMA which is a potent mitogen for human peripheral blood lymphocyte also act as a protein kinase C (PKC) activator which differentiate K652 cells to megakaryocytes. The various changes that occurs during differentiation of K652 cells are: Changes in cell morphology Cell growth arrest Adhesive properties of cell change Expression of markers associatated with megakaryocytes Endomitosis NADPH oxidase complex which is known as a primary source of ROS (Radio active oxygen species), is initiated by PMA. PMA stimulates NADPH ROS (Signalling Molecule) Induction of gene expression is directly related with ROS. The Expression of CD61, a platelet cell marker helps in identifying differentiated cells. The expression of CD61 can be seen on platelets, osteoclasts, macrophages and on some tumour cells, involved in tumour metastasis and in adenovirus infections. Results and Discussion: It was observed that PMA treated slide was stained pink while cells devoid of PMA were stained blue as shown in Pic. 1 2. In PMA treated slide the K562 cells were clearly differentiated to megakaryocytes which suggested that tumour promoter, PMA induced differentiation in K562 cells by signal transduction and expressed by CD61 as shown in picture below. C:UsersmkkaushalPicturescell bio picsmail2.jpg Pic. 1 PMA Treated Cells clearly showing Differentiation to Megakaryocytes C:UsersmkkaushalPicturescell bio picsmail.jpg Pic. 2 Cells Devoid of PMA stained blue in Colour The expression of CD61 was recognised by addition of rabbit anti-mouse IgG antibodies that bind to CD61 antibodies when incubated in presence of alkaline phosphatise anti alkaline phosphatise (APAAP) complex. The cells were stained pink because fast red dye get attached to APAAP so this is how CD61 was expressed in cell treated with PMA. Moreover cells treated with PMA were larger, irregular, in shape and fewer in number as compare to untreated cells. On the contrary, Cells devoid of PMA were much smaller in size than treated cells. Diagramatic Representations of Immunocytochemical Reactions To Detect CD61 PMA Treated Cells PMA Untreated Cells CD61 bound to both treated and untreated cells Then Cells are washed to Remove CD61 unbound Treated with RAM Then RAM binds to APAAP and cells are stained pink in colour. : Mouse Antihuman CD 61 (Primary Antibody) : Rabbit Antimouse IgG (RAM- Secondary Antibody) : Mouse Alkaline Phosphate AntiAlkaline Phosphatase(APAAP Tertiary Antibody)

Permissible Violence in the case of Self-Defense :: essays research papers

In Martin Luther King’s essay â€Å"The Ways of Meeting Oppression† and in the text â€Å"Nonviolence†, the term nonviolence is explained as a technique for social struggle. On the other hand, in the reading â€Å"The Black Panther Party for Self- Defense† it is stated that this social struggle doesn’t always carry the same meaning with the term nonviolence. As I agree with Black Panther’s idea, in my essay, I am going to discuss the extent that the black panthers’ resort to violence is justifiable. According to Martin Luther King, there are three ways that oppressed people cope with oppression; Acquiescence, basically where the oppressed get used to being oppressed. Resort to physical violence and corroding hatred, which would bring momentary solutions and establish additional and more complex problems. Nonviolent resistance, that seeks to create a balance between the acquiescence and violence by preventing the extremes and immoralities of both. In the text â€Å"Nonviolence† the term is explained as â€Å"a set of assumptions about morality, power and conflict that lead its proponents to reject the use of violence in efforts to attain social and political goals.† (p.1) As King implies, those assumptions does not imply a battle between people but a opposition between justice and injustice and by the help of nonviolent resistance the Negro can fight for equality. The hint is to create effective tactics and considering political and cultural conditions, and develop a better plan or strategy. As the rule of capitalism, the rulers’ power depends on the populace’s power. However, the concept of nonviolence challenges the power of rulers through the intentional removal of this co-operation. As Martin Luther King implies; â€Å"Through nonviolent resistance the Negro will be able to noble height of opposing the unjust system while loving the perpetrators of the system.† (p. 139.) From the beginning, the behaviors of the doers are aimed to be changed. However, because of the rising strength of the violence against the Negro had built the foundation for a self- defense movement to achieve liberation for all Black people, which is called â€Å"The Black Panther Party.† For all the Black people, the party wanted freedom, full employment, an end to the robbery of white people, decent housing, education, being held in prison and jails and being tried in a court by a jury of their peer group. When the topic came to the exemption of Black men from military service and an immediate end to police brutality and murder of Black people it also came to the extent the black panthers’ resort to violence is justifiable.

Beyond mass media

Mass media are inherently incompatible with a participatory society because of their mass character, not just because of government control or corporate influence. Mass media should be abandoned and replaced by participatory media organised as networks, such as telephone and computer networks. Los medios de comunicacion son inherentemente incompatibles con una sociedad participativa, debido a su caracter de masas, no solo por el control del gobierno o de la influencia corporativa. Los medios de comunicacion deben ser abandonados y sustituidos por los medios participativos organizados en redes, como el telefono y las redes informaticas. Complaints about the mass media are commonplace. To begin, there is the low quality of many of the programmes and articles. There is the regular portrayal of violence, given an attention out of proportion with its frequency in everyday life. – More generally, most of the mass media give much more attention to bad news–crime, deaths, disasters, wars, etc. –than to positive sides of the human condition. – The mass media frequently create unrealistic fears about criminals, foreign peoples and the like. Las denuncias de los medios de comunicacion son comunes. Para empezar, esta la baja calidad de muchos de los programas y articulos. No es la representacion ordinaria de la violencia, dada una atencion desproporcionada en relacion con su frecuencia en la vida cotidiana. -Mas en general, la mayoria de los medios de comunicacion dan mucha mas atencion a las malas noticias – la delincuencia, muertes, desastres, guerras, etc – que a los aspectos positivos de la condicion humana. -Los medios de comunicacion con frecuencia crean temores poco realistas sobre los criminales, los pueblos extranjeros y similares. â€Å"News† often is more like entertainment than information or education. -News reports, especially on television, are typically given WITHOUT much overt context. The latest events are described, but not what led up to them or caused them. The result is that consumers of the media learn a lot of facts but frequently don't understand how they fit together. â€Å"Context† is the result of the assumptions behind the facts, and this context is all the more powerful because it is neither st ated nor commented upon. â€Å"Noticias†, a menudo es mas como entretenimiento que la informacion o la educacion. -Los informes de prensa, sobre todo en la television, se suelen dar sin mucho contexto manifiesta. -Los ultimos acontecimientos se describen, pero no lo levaron a ellos o les causo. El resultado es que los consumidores de los medios de comunicacion aprenden mucho de los hechos, pero con frecuencia no entienden como encajan entre si. â€Å"Contexto† es el resultado de los supuestos detras de los hechos, y este contexto es aun mas potente, ya que no es ni afirmo ni comentado. Even the â€Å"facts† that are presented are often wrong or misleading. – Powerful groups, especially governments and large corporations, shape the news in a range of ways offering access to stories in exchange for favourable coverage, spreading disinformation, and threatening reprisals. Incluso los â€Å"hechos† que se presentan son a menudo erroneas o enganosas. – Los grupos poderosos, especialmente los gobiernos y las grandes corporaciones, forma la noticia en una gama de formas que ofrece acceso a las historias a cambio de una cobertura favorable, desinformar, y las represalias mortales. This argument suggests that reform of the media, although useful, should not be the goal. Instead, the aim should be to replace mass media by communication systems which are much more participatory. Este argumento sugiere que la reforma de los medios de comunicacion, aunque util, no debe ser la meta. En cambio, el objetivo debe ser la sustitucion de los medios de comunicacion mediante sistemas de comunicacion, que son mucho mas participativo. Replace undemocratic media structure: reemplazar las estructuras de comunicacion democraticos.. The usual approaches Private mass media are often justified as being a vital part of the â€Å"marketplace of ideas. † But, as a way of promoting truth, this so-called market is largely a myth, serving mainly the interests of elites. Los medios de comunicacion privados a menudo se justifican como una parte vital del â€Å"mercado de las ideas. Pero, como una forma de promover la verdad, este llamado mercado es en gran parte un mito, que sirve principalmente a los intereses de las elites. ARGUMENTS AGAINST THE MASS MEDIA (1) Emergencies. The mass media, especially radio and television, can come in handy in emergencies: messages can be broadcast, reaching a large fraction of the population. Emergencias. Los medios de comunicacion, especialme nte la radio y la television, puede ser util en situaciones de emergencia: los mensajes pueden ser transmitidos, alcanzando una gran parte de la poblacion. AGAINST—–But the mass media are not really necessary for emergency purposes. Fire alarms, for example, do not rely on conventional media. Furthermore, network media, including telephone and computer networks, can be set up to allow emergency communications. Pero los medios de comunicacion no son realmente necesarios para casos de emergencia. Las alarmas de incendio, por ejemplo, no confian en los medios de comunicacion convencionales. Por otra parte, los medios de comunicacion de red, incluyendo telefonia y redes informaticas, se pueden configurar para permitir las comunicaciones de emergencia. Actually, the mass media are a great vulnerability in certain emergencies: military coups. Because they allow a few people to communicate to a large population with little possibility of dialogue, television and radio stations are commonly the first targets in military takeovers. Censorship of newspapers is a next step. This connection between coups and mass media also highlights the role of mass media in authoritarian regimes. En realidad, los medios de comunicacion son una gran vulnerabilidad en ciertas situaciones de emergencia: los golpes militares. Porque permiten que algunas personas se comunican a una gran poblacion con poca posibilidad de dialogo, la television y la radio son normalmente los primeros blancos en golpes militares. La censura de prensa es el siguiente paso. Esta conexion entre los golpes de Estado y medios de comunicacion tambien pone de relieve el papel de los medios de comunicacion en los regimenes autoritarios. AGAINST—-Military strength is no defence against a military coup, and indeed may be the cause of one. To resist a coup, network communications are far superior to mass media (Schweik Action, 1992). So, from the point of view of preparing for emergencies, mass media are bad investments. La fuerza militar no es una defensa contra un golpe de estado militar, y de hecho puede ser la causa de una. Para resistir un golpe de Estado, las comunicaciones de red son muy superiores a los medios de comunicacion (Schweik Accion, 1992). Por lo tanto, desde el punto de vista de la preparacion para casos de emergencia, los medios de comunicacion son malas inversiones. (2) Media talent. The mass media allow many people to enjoy and learn from the efforts of some very talented people: actors, musicians, athletes, journalists and commentators. True. But just as many people can enjoy and learn from these talented people without the mass media, for example through audio and video recordings. 2) el talento Media. Los medios de comunicacion permiten que muchas personas puedan disfrutar y aprender de los esfuerzos de algunas personas muy talentosas: actores, musicos, atletas, periodistas y comentaristas. Verdadero. Pero al igual que muchas personas puedan disfrutar y aprender de estas personas con talento y sin los medios de comunicacion, por ejemplo a traves de grabaciones de audio y video. AGAINST—-Furthermore, the mass media suppress access to all but a few performers and contributors. Those who are left out have a much better chance of reaching a sympathetic audience via network media. Por otra parte, los medios de comunicacion suprimir el acceso a casi todos los artistas y colaboradores. Los que se quedan fuera tienen una mejor oportunidad de llegar a un publico mas simpatico a traves de los medios de comunicacion de la red. Richard Schickel (1985) points out that the celebrity is a twentieth-century phenomenon, created especially by movies and television. He describes a culture of celebrity, in which people strive to be well known, even if this is only because they have appeared on the screen. The culture of celebrity, he argues, is undermining many traditional practices. For example, politicians are sold on the media in terms of image rather than policies. Richard Schickel (1985) senala que la celebridad es un fenomeno del siglo XX, creado especialmente por el cine y la television. El describe la cultura de la celebridad, en la que las personas se esfuerzan por ser bien conocido, aunque esto es solo porque han aparecido en la pantalla. La cultura de la celebridad, sostiene, esta socavando muchas de las practicas tradicionales. Por ejemplo, los politicos se venden en los medios de comunicacion en terminos de imagen, mas que politicas. (3)Large resources. The mass media command enormous resources, both financial and symbolic. This makes it possible for them to pursue large or expensive projects: large-budget films, special investigative teams, in-depth coverage of key events. 3) los recursos grandes. Los enormes recursos de los medios de comunicacion de masas de comando, tanto economicos como simbolicos. Esto hace que sea posible para ellos para perseguir grandes o costosos proyectos: peliculas de gran presupuesto, equipos especiales de investigacion, la cobertura en profundidad de los principales acontecimientos. AGAINST—-Actually, large-scale projects are also possible with network systems. They simply require cooperation and collaboration. For example, some public domain software (free computer programmes) is quite sophisticated and has been produced with the help of many people. In centralised systems, far-reaching decisions can be made by just a few people. In decentralised systems, greater participation is required. En realidad, los proyectos a gran escala tambien son posibles con los sistemas de la red. Simplemente se requiere de la cooperacion y la colaboracion. Por ejemplo, algunos programas de dominio publico (programas informaticos gratuitos) es bastante sofisticado y ha sido elaborado con la ayuda de muchas personas. En los sistemas centralizados, decisiones de gran alcance se pueden hacer unas pocas personas. En los sistemas descentralizados, se requiere una mayor participacion. These four possible arguments for retaining mass media, in some reformed and improved form, actually turn out to be arguments against mass media. ABSTRACT AGAINST: – The mass media are not necessary for emergencies and are actually a key vulnerability to those who would take over a society. -The mass media are not necessary to enjoy and benefit from the talent of others, and they foster an unhealthy emphasis on image. -Finally, although the mass media can undertake large projects, such projects can also dev elop through network media, but in a way involving participation rather than central direction. Estos cuatro argumentos posibles para retener los medios de comunicacion, de una forma reformada y mejorada, en realidad resultan ser argumentos en contra de los medios de comunicacion. RESUMEN EN CONTRA – Los medios de comunicacion no son necesarios en caso de emergencia y en realidad son una vulnerabilidad clave a los que se haria cargo de una sociedad. -Los medios de comunicacion no son necesarios para disfrutar y aprovechar el talento de los demas, y fomentar un enfasis enfermizo en la imagen. -Por ultimo, aunque los medios de comunicacion pueden llevar a cabo grandes proyectos, estos proyectos tambien pueden desarrollarse a traves de los medios de comunicacion de la red, pero de una manera que implica la participacion en lugar de la direccion central. PARTICIPATORY MEDIA AGAINST–Mass media are inherently corrupting. A small number of owners and editors exercise great power over what is communicated to large numbers of people. -Mass media should be replaced by participatory media organised as networks, such as telephone and computer networks. Los medios de comunicacion son inherentemente corrompiendo. Un pequeno numero de propietarios y editores ejercen un gran poder sobre lo que se comunica a un gran numero de personas. – Los medios de comunicacion deben ser sustituidos por medios participativos organizados en redes, como el telefono y las redes informaticas.

cyber ethics essays

cyber ethics essays The article I choose was written by Jennifer Howland and encompasses her familys views to the Recording Industry Association of America lawsuit of 261 people, including teens, elderly, occasional downloaders, and frequent downloaders of music. Mrs. Howland has two daughters whose life is based around the computer. Everything from socializing, to gaming, to music listening. Her daughters share music files with their friends like most teens, but after the lawsuit from RIAA Mr. Howland had to end usage of P2P programs which enabled the girls to share music with their friends. He noted that one of the teens sued had to settle for $2,000, and with one of his daughters off to college soon he has no room for financial missteps. Jennifer then notes that she ordered here children not to download for fear of not getting sued, not because she thought the action its self was wrong. She was telling them that it wasnt the principle thats important, its the consequences themselves. She then raises the issue that the music industry tries to increase our sense of compunction by arguing that downloaders are, in effect, stealing musicians livelihoods. Yet, many artist including David Bowie, and countless smaller band tend to believe that it creates a medium for further exposure. With the smaller band you can sample a song or two, and if you like it find out where they are touring next, or purchases the CD outright. I tend to agree with this style of though. I have downloaded my share of music, mostly music that doesnt have a market in this area just yet, but when I stumble across something new that I like, I look into touring dates, and album info. I believe that this is a great way to increase exposure of bands that get little radio play, or just havent made the leap to being signed by a record label. As far as the bigger bands out there that have Made It what ...

So You Want to Study Abroad (6 Things to Consider)

So You Want to Study Abroad (6 Things to Consider) So You Want to Study Abroad (6 Things to Consider) More and more people choose to study abroad these days. And why not? You get to travel, meet new people, learn about a different culture and earn a college degree at the same time! But studying abroad requires a bit of preparation, so make sure you’ve considered the following factors†¦ 1. Length of Placement Different placements are available depending on how long you want to spend studying abroad. This ranges from a few weeks or a single semester, to full degree programs for those who want to really throw themselves into an overseas experience! 2. Where to Go This is the big one! Where should you study? Some of the most common places for U.S. students to study are in Europe, but it’s possible to study almost anywhere in the world, with other popular destinations including Canada, Mexico, Japan, China and Australia. As well as your personal interest in wherever you decide to study, practical factors to consider include your familiarity with the language (both of the country and the language of instruction), the lifestyle and culture of the place where you’ll be studying, and the tuition and living costs involved. If you really cant decide, throw a dart at a world map and see where it sticks. [Image: Mason Vanks Maps/wikimedia] 3. The Program Once you know where you want to study and the length of placement you’d prefer, it’s time to start looking at different programs. If you’re simply studying abroad for part of your degree, your best option will usually be to apply for a program run by your college. If you want to study abroad for your entire degree, you’ll have to research your chosen destination and apply as an international student. 4. Funding If you have the money to pay for your studies already, you can skip this one. But many of us with dreams of studying abroad would appreciate a little financial help, so it’s worth researching whether funding is available. 5. Entry Requirements and Qualifications The application process for studying abroad differs from institution to institution, so make sure you check carefully and understand the requirements. This will include having the academic qualifications necessary to study your chosen course, as well as providing a resume, a letter of recommendation and possibly proof that you can speak the language of instruction. Some colleges and universities also set admissions tests. The most important thing is starting the application early enough, as that will give you enough time to organize everything before you’re due to begin your studies! 6. Travel Arrangements The final step, once you’ve been accepted on a course, is to organize the travel. And there’s lots to do before booking your flights, including sorting out a visa, making sure you have a valid passport and working out your living arrangements for once you arrive. As with the entry requirements, the key here is knowing what you need to do before travelling to the country where you’ll be studying, as well as leaving plenty of time to make the arrangements. And then its just a case of Bon voyage!

Most important figure in American History Essay

Most important figure in American History - Essay Example "And it may be truly said, that never did nature and fortune combine more perfectly to make a man great" - Jefferson (Hawkinson, 2005: 243). Washington served in the American Revolutionary War as a commander in the Continental Army. He had been appointed in 1775 as Commander-in-Chief of the American revolutionary forces by the Continental Congress. The next year it was due to him that the British were driven out of Boston. He also failed New York City that same year plus went across the Delaware River in New Jersey. He astonished and defeated the enemy units. It was because of his tactics that the Revolutionary forces were able to capture the two major British combat armies at Saratoga and Yorktown. When the war was over Washington went back to his private life which prompted the surprised King George III to say, "If he does that, he will be the greatest man in the world" (Higginbotham, 2001: 221). Washington presided over the Philadelphia Convention which was responsible for drafting the Constitution of America due to there being mutual discontent with the Articles of Confederation. This happened in 1787 and two years later he became the president of the country (Freidel, 1994). Following his presidency he set up several customs and a using of the novel government's executive department. Washington attempted at creating a country that would have the capability of surviving in a world that was going down due to the ongoing war between Britain and France. He was in favor of plans for building a powerful central government which could be done if funded through the national debt, if there was an implementation of an efficient tax mechanism, and also through the creation of a national bank. Instead of going for war, Washington peacefully negotiated with Britain through the Jay Treaty in 1795. Washington was in favor of the programs organized by the Federalist Party but never actua lly joined the group. Washington is a great figure in American history and is perceived to be the country's identification plus said to be republicanism with regard to his practices. The early American politicians admired Washington greatly. He was given the first award of Congressional Gold Medal with the Thanks of Congress. Washington expired in the year 1799. Several scholars have named him as one of the greatest Presidents of United States. Biography Washington was born in the February of 1732 (Leibiger, 2001). His father passed away when he was 11 and his half brother assumed the part of his father. Washington experienced a lot of protectiveness and demand from his mother who did not allow him to join the British navy as Lawrence, his brother, wanted. When Washington was 16 he went on to live with Lawrence on Mount Vernon. He had his schooling at Colonial Virginia but did not go to college. In 1749 Washington became the surveyor for Culpepper County, Virginia. This happened following a trek for Lord Fairfax. From 1752 till 1758 he was in the military after which the Virginia House of Burgesses elected him. His speeches were in opposition to Britain's policies. Then he even became a leader in the Association. From 1775-1783 Washington led the Continental Army during the American Revolution after which, in 1787, he was made the president of the Constitutional Convention. Although

Environmental Politics Essay Example | Topics and Well Written Essays - 2500 words

Environmental Politics - Essay Example Environmental politics can also play an important role in global governance by finding new ways to hold individual governments responsible for their polluting activities. Turner explains this phenomenon in his article on measuring the carbon footprint. He argues that recent approaches towards measuring carbon footprints can be a useful means of assessing the responsibility of each state for reducing and controlling carbon emissions within their jurisdiction. Furthermore, the new measures also provide greater opportunity for deeper engagement and collaboration between states to develop strategies for reducing carbon emissions in various countries.Despite the promises of environmental politics bringing about greater cooperation, the reality also presents a picture characterized by disagreement and conflict. These fears are described by Skjaerseth, Bang, and Schreurs. The authors chart the course of environmental protection negotiations between the United States and the European Union a nd highlight the obstacles which have prevented a global consensus on emission reduction and climate change. Their findings show that environmental politics can only be an effective test of global governance to the extent that there is congruity or integration between the legislative organs and environmental bodies of the member states. If there is a contradiction between the ideals of environmental politics and what global environmental organizations are empowered to do, then productive results cannot be achieved.